Theory Experiment
After the human practice part, we had a profound discussion about changing the project. Finally, we changed our project into the treatment for cancer in this month.
On July 5, we designed our Single-Guide RNA (sgRNA) which could specifically bind to the mutant BRAF gene.
On July 8, we started to culture the melanoma cell lines A375 and G361.
On July 9, we cultured the bacteria containing our plasmid containing our sgRNA and Cas9 protein.
On July 11, we extracted genome from A375 and G361 cell lines to check if the cell lines had BRAF V600E mutation. We extracted the plasmids to prepare for cell transfection.
On July 13, we transfected the cells with our plasmid.
On July 14, we assigned the task of writing different parts of our wiki to everyone and started writing our abstract, description and design of our wiki.
We cultured the cell lines.
On July 20-21, we submitted 4 parts.
On July 26, we cultured the cells in a 6-well plate and transfected the cells in order to measure cell migration by wound healing assay.
On July 27, we seeded the cells in a 96-well plate and trasnfected the cells in order to measure the cell proliferation by Transdetect Cell Counting Kit (CCK). We planned to scratched wounds across the cells but failed, for G361 cells didn’t
cover the dish.
On July 28-30, we stained the cells to detect the results of CCK assay. We cultured the cells.
On July 31, we finished writing our first draft of our abstract, description and design.
Human Practice
Attended the 19th International Botanical Congress.
On July 10th, we met up with Southern University of Science and Technology iGEM team.
From July 24th to July 30th, we went to Bali island to teach teenager “How to keep healthy”.
On July 30th, we delivered a public speech about iGEM, Synthetic Biology and Melanoma in Shenzhen Bookcity and gave out questionnaires while the speech was going on.
